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KMID : 0903520080510010011
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2008 Volume.51 No. 1 p.11 ~ p.16
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Resveratrol Extraction from Grape Fruit Stem and its Antioxidant Activity
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Cho Cheol-Hee
Kim So-Young
Yoo Gui-Jae
Son Min-Hee
Park Keun-Hyoung
Lim Byung-Lak
Kim Dong-Chung
Chae Hee-Jeong
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Abstract
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The extraction conditions for resveratrol production from grape fruit stem, which is a by-product of grape processing, were optimized to develop high-functional grape-based products. Additionally, the bioefficacy of grape fruit stem extract (GFSE) as an antioxidant agent was evaluated. Resveratrol was extracted using various experimental conditions such as extractant type, extractant concentration, raw material-extractant ratio, extraction time and temperature, and the results were analyzed using a statistical program (SPSS). The resveratrol yield was the highest when 80% ethanol with a raw-material-extractant ratio of 1 : 10 (w/v) was used. In addition, the optimal temperature and time were selected as 60oC and 90 min, respectively. When the antioxidant activity was analyzed and expressed as DPPH radical scavenging activity and SOD-like activity, the antioxidant activity of GFSE was higher than that of BHT, BHA and L-ascorbic acid. Finally, it was found that GFSE could be used as a raw material for the production of high antioxidant agents.
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KEYWORD
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antioxidant activity, extraction condition, grape fruit stem, resveratrol
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